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新建 Mygeninsc-576


Mygenin sc-576 Qifferentiatian of myagenic cells is regulated by multiple positively and negatively acting factors. Gne well cf}aracteri}ed family of helix-loop-helix }HLH) proteins known to

play an important role in the regulation of muscle cell development includes MyoQ, myoyenin, Myf-5 and Myf-6 salsa designated MRF-4 or herculin). Gf interest, most muscle cells express either MyaQ or Myf-5 in the committed state, but when induced to differentiate, all turn an expression of myageni}、一 MyoQ transcription factors form heteradimers with products of a mare widely expressed family of hHLH genes, the E family which consists of at least three distinct genes: E2A, IF2 and H 田 MyaQ-E heteradimers hind avidly to consensus iCANNTG) E hox target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Genetic locus: MYOG (human) mapping to 1q32.1; Myag (mouse) mapping to 1 E4

myogenin (M-225) is a rabfcit palyclanal antibody raised against amino acids 1-225 representing full length myagenin protein of rat origin.

Each vial contains 20} pg IyG in 1.D ml of PB5 with<}.1'}} sodium aide and 口 1'}} gelatin. Available as TransCru} reagent far Gel Supershift and ChIP applications, sc-576 X,2}} pg}}.1 ml

myagenin (M-225) is recommended for detection of myayenin of mouse, rat and human origin by Western Blotting (starting dilution 12}}, dilution range 1 门}}-1:1 口 oo), immunaprecipitatian [1-2 py pe 门 0 口·5 口口 pg of total protein [1 ml of cell lysate)[, immunafluorescence [starting dilution 1:5}, dilution range 1:5 口·1:5 口}) and solid phase ELISA[starting dilution 1:3}, dilution range 1:3}-1:3 口口 o). myogenin (M-225) is also recommended far detection of myayenin in addibanal species, including equine, canine, bovine and porcine 5uitahle for use as control antibody far myayenin siRNA [h): sc-29402, myogenin siRNA (m): sc-35992, myoyenin shRNA Plasmid (h): sc-29402-SH myogenin shRNA Plasmid (m): sc-35992-SH, myoyenin shRNA [h) Lentiviral Particles: sc-29402-V and myoyenin shRNA [m) Lentiviral Particles: sc-35992-V. myogenin (M-225) X TransCru} antibody is recommended for Gel 5 叩 ershift

and ChIP applications. Molecular Weight of myagenin: 34 kDa. Positive Controls: 5JRH30 cell lysate: sc-2287, myogenin (h3): 293T Lysate: sc-177592 or RQ whale cell Ivsate. Store at 40 C, ''''Q0 NOT FREEZE"'. Stahle for one year from the date of shipment. Non-f}a}ardous. No MSQS required.

}m;u:}eriii ";ha-1251 s._-515 rltieslern L .'.: analysis u' }m;u:}eriii axpressiur r lion-:ransfec:ac“一 1'7752 ;Ai. f}u}nan rrr;c}.cen r lrars'acleJ s._-115551 ";B; 2Cr3T arJ SJF}3G iCi vrliu a cell lysales rrrru:lerin i'.9-225;: sc-515. ."feslern Llul aralys s 。 ‘rrrru:lerin enprassiun in nun-lransfecleJ 2"3T: s‘一 117751 iAi. human rrrru:lerir lransfecleJ 2"3T S‘一 1i75"Q i8i anc SJR}3G ":C'} vrli}l=cell ;sales

1. Guttridye, D.C., et al. 1997 一丁 hromhin causes a marked delay in skeletal myagenesis that correlates with the delayed expression of myagenin and p21CIP1}WAF1.J. Bial.Chem.272:24117-2412 2. Bishal 已一 et al 一 2D 口口 The 2'-5' oliyaadenylate,}RNase L}RNase L inhibitor pathway regulates bath MyaD mRNA stability and muscle cell differentiation 州 oL Cel 卜 Bial 20- 4959-4969 3 州 uralikrishna. B.. et al. 2D 口 1. Distinct changes in intranuclear lamin 周 C oryani}atian during mya 匕 last differentiation. J. Cell 5ci. 114: 4 口}1-4011 4. Suelves, M.et al. 2 口 D2.户 lasmin activity is required far myagenesis in vitro and skeletal muscle regeneration in vivo. Blood 99: 2835-2844 5. Kumar, N., et al. 2}}3. Development of Insulin resistance and reversal by tfua}alidinediones in C2C12 skeletal muscle cells. Biochem. Phannacal. 65 249-257 6. Roth, J.F., et al. 2003. Differential role of p3}} and CBP acetyltransferase during myogenesis: p3 口} acts upstream of MyaD and Myf-5. EMB})一 22 5186-5196. 7. Ohkawa,丫 and Yashimura,s 一 2 口口 7. Myoyenin and the SWI,}SNF ATPase Bry1 maintain myayenic gene expression at different stages of skeletal myagenesis. J. Bial. Chem. 282: 6564-6570. 8. Du, 5.J .et al. 2 口 D8. Heat-shack protein 日口(G1 is required far oryani}ed myofibril assembly in skeletal muscles of }ehrafish embryos. Proc. Natl. Acad 5cilJSA 1 口 5:554-559 9. Kottlors,州 and Kirschner, J. 2}1 口[levated satellite cell number in Duchenne muscular dystrophy. Cell Tissue Res. 34}: 541-548 10 口 Mallappa, C., et al. 2}1 口丁 he expression of myagenic microRNAs indi-

rectly requires protein arginine methyltransferase }Prmt)5 but directly requires Prmt4. Nucleic Acids Res. 39: 1243-1255. Myod sc-760 Qifferentiatian of myagenic cells is regulated by multiple positively and negatively acting factors. Gne well cf}aracteri}ed family of helix-loop-helix }HLH) proteins known to play an important role in the regulation of muscle cell development includes MyoQ, myoyenin, Myf-5 and Myf-6 salsa designated MRF-4 or herculin). Gf interest, most muscle cells express either MyaQ or Myf-5 in the committed state, but when induced to differentiate, all turn an expression of myageni}、一 MyoQ transcription factors form heteradimers with products of a mare widely expressed family of hHLH genes, the E family which consists of at least three distinct genes: E2A, IF2 and H 田 MyaQ-E heteradimers hind avidly to consensus iCANNTG) E hox target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes. genetic Focus 州 YuUI }humanl mappin9 to 11p15.4; Myod1 }mause) mapping to7B4 MyaQ }M-318) is a rafcbit palyclonal antifcady raised against amino acids 1-318 representing full length MyaQ protein of mouse origin.

Each vial contains 2D 口 pg IgG i}门一 Dmlof 户 BS with(o.}%sodium aide and 口一 1`}} gelatin Available as ayarose conjugate far immunoprecipitation, sc-76} AC 5 口} pg/口 25 ml agarase in 1 ml Available as TransCru} reagent for Gel 5upershift and ChIP applications, sc-76 口 X, 2}} pg}}.1 ml

MyoD iM-318) is recommended far detection of MyaQ of mouse, rat and human origin by Western Blotting istarting dilution 1:200, dilution range 1 门口口·1:1}}口}inununoprecipitation [1-2 py pe 门 0 口·5 口} py of total protein i1 ml of cell lysate)], inununafluarescence istarting dilution 1:5}, dilution range 1:5 口·1:5 口}) and solid phase ELISAistartiny dilution 1:3}, dilution range 1:3}-1:3 口口 o). MyoD iM-318) is also recommended far detection of MyaQ in additional species, including equine, canine, bovine and porcine 5uitahle for use as control antibody for MyaQ siRNA ih): sc-35990,0 MyoD siRNA im): sc-35991, MyoD shRNA Plasmid ih): sc-35990-SH, MyoD sf}RN} Plasmid im): sc-35991-SH, MyoD shRNA ih) Lentiviral Particles: sc-35990-1 and MyaQ shRNA im) Lentiviral Particles: sc-35991-V. MyaQ iM-318) X TransCru} antibody is recommended far Gel S 叩 ershift

and ChIP applications. Molecular Weight of MyaQ: 45 kDa. Positive Controls: A-673 cell lysate: sc-2414, L8 cell lysate: sc-3807 or SolB cell lysate: sc-2249

Stare at 40 C, ''00 NAT FREE2E"'. 5tahle for one year from the date of shipment. Non-f}a}ardous. No MSQS required.

h:1};c}.C i'.9-3'2}. sc-i}G.'v'daslerr Llul aiiekrsis of '.9};c}.C a.}prass c}.ii 川 LS IA;. =}-C i 3 };B;. to 旧};C; enc C2C' c iii raiu a cell ysa'.as arJ rric}.use skelalel }n}_scle'.issue a.}lracllEi

Peschiarali, A., et al 一 2D 口 2. MyaQ induces apaptosis in the absence of RB function through a p21waF}-dependent re-lacali}atian of cycli}}}Cdk complexes to the nucleus. }ncogene 21: 8114-8127. 2. Mooch, R., et al. 2D 口 3 丁 he homeodamain protein Barx2 promotes myagenie differentiation and is regulated by myogenic regulatory factors J. Biol. Chem. 278: 8269-8278 3. Mal, A. and Harter, M.L. 2 口口 3. MyoQ is functionally linked to the silencing of a muscle-specific regulatory gene prior to skeletal myogenesis. Proc. Natl. Acad. Sci. USA 10}: 1735-1739. 4. Lees, S.J 一。t al. 2D 口 6 匆 e-associated decrease in muscle precursor cell differentiation、一 Am. J 一 Physial.已 ell 户 hysiol 一 29}已 6 口 9-C615. 5. Li, 2.Y. and Yang, J 一 2D 口 7. Sequential recruitment of PCAF and BR 已 1 contributes to myogenin activation i}门 2-0-Tetradecanoylphorhal-l3acetate-induced early differentiation of rhahdomyosarcoma-derived cells J 日 ial Chem282: 18872-18878 6. Shi. X. and 已 arry, D.J 一 2}1 口 Myogenic regulatory factors transactivate the Tceal7 gene and modulate muscle differentiation. Biachem. J. 428: 213-221 7. Micheli, L.et al 一 2 口 1D. PC4}Tis7,}IFRD1 stimulates skeletal muscle regenoration and is involved in myahlast differentiation as a regulator of MyoD and NF}cB. J. Biol. Chem. 286: 5691-5707 8. 5tappani, E., of al. 2011. Point mutated cavealin-3 form (P1}4L) impairs myahlast differentiation via Akt and p38 signalling reduction, leading to an immature cell signature. Biachim. Biaphys. Acta 1812: 468-479.

For research use only, not for use in diagnostic procedures

See our web site at www.scbt.cam or our catalog for detailed protocols and support products


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