当前位置:首页 >> 语文 >>

blood cell 血液


HISTOLOGY & EMBRYOLOGY
Teaching PPT

BLOOD

一、CONSTRUCTION
Plasm (I.M.) : 55% Hemocytes(cells):45% erythrocytes leucocytes blood platlets * hematocrit(pack

ed red cell volume,红细胞压积): 45%
Plasm
Leucocytes & Platlets

Erythrocyte

*using anticoagulants

1. Plasm
90% water 10% others : ? plasma pr.
? ? ? ? ?

Serum

albumin components same fibrinogen as that of plasm but fibrinogen enzymes Ig. etc.
serum

Lipid hormones Vitamins inorganic salts metabolites

Blood clots

BLOOD CELLS
?blood smear stains w/ Wright or Giemsa

Erythrocytes: 4.2~5.5×1012/L(M) 3.5~5.0×1012/L(W) *Hb: 120~150g/L(M);105~135g/L(W) Leukocytes: 4~10×109/L neutrophils 50~70% Granulocytes eosinophils 0.5~3 % basophils 0~1% Agranulocytes lymphocytes 20~30% monocytes 3~8% Platelets: 100~300×109/L

Erythrocyte
?

?
? ?

biconcave discs, 7.5~8.5μm,flaxible no nuclei,no organelles rich in Hb:105~150g/L blood group (ABO; Rh…): dependent on the agglutinogen (on the membrane)

Pathology
?

Anemia:R.B.C < 3×1012/L or Hb< 100g/L megaloblastic anemia(巨细胞贫血) : >9 μm

iron deficiency animia: (缺铁性贫血) <6 μm
?

Osmotic stress abnormal: hypotonic(低滲): RBC swelling &hemolysis (remain ghost血影); hypertonic (高滲): RBC collapsing

? Reticulocytes
? 0.5~1.5% ? containing ribosomes & Mit. (stained by brilliant cresyl blue) ? increase in anemia ? Indicator of hematopoietic capacity of the bone marrow.

? Function
Binding & transporting O2 and CO2

In lungs

In organs & tissues

CO2+HbO2

HbCO2+O2

Leukocytes
?
? ? ?

Spherical, nucleate & colorless
Contain specific or nonspecific gralules

Amoeboid movement
Phagocytosis except

for lymphocytes
?

Involved in defense

against foreign materials

Leukocytes: Granulocyte

Agranulocyte

4~10×109/L neutrophil 50 ~70% eosinophil 0.5~3 % basophil 0 ~1% lymphocyte 20 ~30% monocyte 3 ~8%

A. Neutrophils
? D=10~12μm;

? Nuclear: polymorphous 2~5 lobes increase w/ the age of the cells. ? Specific Granules: 80%, fine (0.3~0.4μm ) neutrophilic (light reddish) ? Azurophilic granules: 20%, larger, purple
(嗜天青颗粒)

E.M.
S.G.: medium-dense containing A.K.P, phagocytins lysozymes ? A.G.: lysosomes containing A.C.P. peroxidase
?

Function
Mobility(chemotaxis): Attracted by polymorphonuclear chemotactic factor (released by macrophages) ? Phagocytose & destroy bacteria: the number of leukocytes↑ acute the % of neutrophils↑ bacteria infection ** cell mark: peroxidase and CD15
?

B. Eosinophilis
?D = 10~15μm; ?Nu.: 2 ~5 lobes ?S.G.: uniformity, large (0.5~1.0μm ), bright red or orange (acidophilic)

?

S.G.:

E.M.

?

Lysosome :ovoid, w/ granule matrix & a elongated crystalloid core Containing: acid phosphatase, peroxidase, histaminase.

Function
? ? ? ?

*

Chemotaxis: attracted by chemotactic factor from the mast cells Phagocytize: foreign bodies, Ag-Ab complex Release histaminase: inactivate histamine for weakening allergic reaction Antiparasite It is increased in patients infected w/ allergic reaction or parasite

C. Basophilics
? D = 10 ~ 12μm ? Nucleus: irregular or S-shaped ? S.G.: large (various size), basophilic (dark blue), cover nuclear, distribute unevenly & metachromasia

EM & FN
? E.M.: S.G. w/ fine grains, containing heparin, histamine, leukotriene ? F.N.: anticoagulate responsible to allergic reaction

D. Monocytes
Largest, 14~20μm, bluish-grey staining ? Nu.: kidney- or horseshoe-shaped w/ delicate net-like Chromatin ? Cytoplasm: abundant, gray blue, w/ fine azurophilic granules
?

E.M :
A.G.: lysosomes (peroxidase, A.C.P., non-specific esterase); Phagocytic vacuoles

F.N. :
amoeboid movement & obvious chemotaxis ?Phagocytosis (mononuclear phagocytic system, MPS) ?differentiate into macrophage (out B.V.) **mark: CD14
?

E. Lymphocytes
?3 types: small
6~8μm; medium-sized 9~12μm; large 13~20μm ?Small lymphocyte(90%): LM: Round or ovoid; Nu.: sphericity w/ indentation, condensed chromatin, dark staining. Cytoplasm: scanty, as a thin rim in light-blue w/ azurophilic granules lacking proxidase

Large lymphocyte

medium lymphocyte

small lymphocyte

Classification & Function
Classification* function# T-Cell: 75% cellular immunity B-Cell: 10-15% humoral immunity large granule lymphocyte (K & NK cell) * based on the source, characteristics on cellular membrane, life & function # immunological defence

Blood platelets
? Megakaryocyte’ s

fragment, in clumps ? LM: 2~4μm,disc-like or irregular, anucleated. Two regions: ① Granulomere: centrallylocated, purple-blue granules. ② Hyalomere: peripherally located, in light-blue.

E.M.:
① cell coat: rich in glycoproteins associated to platelet adhesion; ②M.T. system: a. opening tubule system: to increase the surface area for platelet adhesion. b. dense tubule system: sER , support, collect Ca 2+ and synthesize prostaglandin

③actin-like M.F: mobile. ④ granules: a. αgranules (special granules): containing blood clotting factors (12 types found so far), fibrinogen & acidic hydrolases. b. dense granules: serotonin (5-HT), ADP, ATP, Ca2+ & adrenine

Function
1. Hemostasia: collagenous fiber in injure area platelet release serotonin constraction of b.v. slow & stop bleeding. 2. Coagulation: thrombogen

coagulation factor III
Fibrinogen

thrombase

Fibrin hemocyte coagulation 3. Protect blood vessel endothelium, repairing endothelium, prevent atherosclerosis(动脉硬化)

二、Hematopoiesis
Hematopoietic organ yolk sac (early embryo) →Liver / spleen (6w—5m) → bone marrow (4m and after) ? Structure of bone marrow red bone marrow: hematopoietic tissue + blood sinusoid yellow bone marrow: adipose tissue + immature hemocyte
?

Red bone marrow
? hematopoietic tissue: reticular c.t. +hemocytes in various stage, macrophages, adipocytes, mesenchymal cells ? blood sinusoid: discontinuous endothelia, incomplete BM

Hemopoietic stem cell (HSP) and Hemopoietic progenitor cell (HPC)
Hemopoietic stem cell self-replication powerful proliferation potency multi-differentiation Potency Mark:CD34, CDw90 Hemopoietic progenitor c. Unipotential or bipotential Produce matrue blood c. Erythron granulocyte series monocyte series megakaryocyte series

* Lymphocytes derived from lymphatic organs & tissues

Pb:原红

Be:早幼红

Erythrocyte series

Pe:中幼红

晚幼红

Mb:原粒

Pm:早幼粒

Mb

Neutrophilic series

M,Ma,Mb:中幼粒 M1:晚幼粒

Morphologic criteria of haemopoiesis

① Stages: the original stage the infant stage the mature stage ② Cell volume:↓, megakaryocytes:↑ ③ cytoplasm: ↑,ratio of Nu./ cytoplasm:↓

④ Nucleus: size↓(RBC: abesent; megakaryocytes↑) nucleoli: number & size↓, until disappear chromatin: fine & scanty → coarse & dense; staining: pale → deep ⑤ S.G in granulocytes & Hb in erythrocytes:↑ other organelles in red cell↓until disappear ⑥ mitotic ability:↓, until disappear(but lymphocyte)

Question

BYE

BYE


相关文章:
血液分析
4.1 血细胞分析仪 4.1.1 血细胞分析仪概述 1.概念 血细胞分析仪(Blood cell analyzer,BCA)是指对一定体积全血内血细胞异质性进行 自动分析的临床检验常规仪器。...
犬猫血细胞和血小板直方图[1]
二、 白细胞体积分布直方图 (White blood cell distribution histogram) 血液经溶血剂处理后, 白细胞失水皱缩, 各群细胞间体积差异增大, 仪器中计算机根据其体积大小...
血液
血细胞分为三类:红细胞、白细胞、血小板。 1、 红细胞红细胞( erythrocyte, red blood cell) 直径 7~ 8.5μ m, 呈双凹圆盘状,中央较薄( 1.0μ m) ,...
血液由血浆和血细胞组成
血细胞分为三类:红细胞、白细胞、血小板。 1、红细胞 红细胞(erythrocyte,red blood cell)直径 7~8.5?m,呈双凹圆盘状,中央 较薄(1.0?m),周缘较厚 血液 ...
实验二、血液常规检验
(二) 、红细胞计数 红细胞计数(red blood cell count,RBC)是指计算每升血液内所含红细胞的数目。红细 胞计数的方法有显微镜计数法、血沉管计数法、光电比色法...
血液分析仪概述
第一节概述 血液分析仪(Hematology Analyzer),又称血细胞分析仪(Blood Cell Analyzer),也可称为血细胞计数仪 (Blood Cell Counter),主要用于检测血液标本,能对...
临床检验基础第一章血液的一般检验
第一章血液的一般检验 单项选择题 1.成人静脉采血,采血的部位通常是 A.手背...3.红细胞体积分布宽度(red blood cell volume distribution width,RDW)是反映...
在血液化验单上,“Hb”表示的是( )_答案_百度高考
解:A、红细胞的英文是Red blood cell,简写为RBC,A错误; B、血红蛋白的英文是Hemoglobin,简写为HB,因此在血液化验单上,“Hb”表示的是血红蛋白,B正确; C、血小...
血液基础知识
3.血液细胞分析的检测项目 细胞分析的检测项目 .血液细胞分析 (1). 白细胞检测 WBC(White Blood Cell count): 白细胞计数: 单位体积全血白细胞数,(×103/?L...
血液分离技术
血液分离技术_基础医学_医药卫生_专业资料。第十八章 血细胞分离技术 (Separation technique of the blood cell) 一、原理 在体外进行细胞免疫检测时, 首先必须进行...
更多相关标签:
血液透析 | 血液循环 | 血液病有哪些 | 血液净化 | 全国血液净化登记系统 | 血液病 | 血液粘稠 | 血液 电视剧 |